Composite

Part:BBa_K3089017:Design

Designed by: Aiai Dong   Group: iGEM19_Greatbay_SCIE   (2019-10-15)


rBalcp19K-linker-mfp5-His fusion protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 274
    Illegal PstI site found at 23
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 274
    Illegal PstI site found at 23
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 274
    Illegal XhoI site found at 244
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 274
    Illegal PstI site found at 23
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 274
    Illegal PstI site found at 23
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Redesign the sequence, making it suitable for protein expression


Source

rBalcp19k - barnacle cement protein (Cui, Ren et al. 2017; Kamino 2013)

References